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Laboratory of Biotechnology and Bioanalysis Washington State University

WSU Laboratory for Biotechnology and Bioanalysis (LBB)

 

Derek at the PacBio Machine

Welcome! The Laboratory for Biotechnology and Bioanalysis (LBB) began at WSU back in the early 1990’s. This core was established as a service center and to provide access to instrumentation that proved to be cost prohibitive for individual labs to purchase on their own. Over the past 20 years, with the assistance of the Center for Reproductive Biology, M.J. Murdock Charitable Trust, NIH and others the core lab has evolved into a state of the art service facility.

 

The core lab is directed by Dr. Michael Griswold in the School of Molecular Biosciences and currently has three full-time personnel. The facilities are housed in two separate laboratories in the Biotechnology and Life Sciences Building (rooms 127 and 227). We have a multitude of services and instrumentation available in the fields of molecular biology and genomics. If you have any questions about our services or facilities please feel free to ask.

Sincerely, Derek Pouchnik
Co-Director


New Procedures and Policies During the COVID-19 Pandemic

As WSU researchers slowly begin a return to research activity we are excited to be able to be here to help support your research projects. In order to do so safely we have some procedure and policy changes for our users to follow:

  • Lab Access – BLS is currently locked to researchers outside our building. We are happy to coordinate sample drop off and reagent pickup at the BLS entrance. However, if you require access to LBB please contact us with your request for access, and if necessary access can be provided through WSU key card.
  • Disinfectant/Hand Sanitizer – There is disinfectant and hand sanitizer located outside our lab, as well as within. Please feel free to use these items as well as using our sink for washing your hands if you wish. We will be sanitizing common use areas and instrumentation frequently. Prior to using common use instruments such as the nano-drop spectrophotometer and Diagenode Bioruptor, lightly spray and wipe down with 70% EtOh to disinfect before and after each use.
  • Social Distancing – Central to CDC guidelines for fighting Covid-19 is practicing social distancing. Please continue to practice social distancing when within the lab. Only personnel needed to perform the experiment/measurement should be present. If you are waiting for an instrument there is ample space in the common area on the second floor outside our lab.
  • Facial coverings/masks – Masks are required and can be obtained from WSU University stores. Alternatively, Ferdinands is selling custom WSU Cougar cloth facemasks that look pretty nice!

Special Instructions

In order to minimize foot traffic through LBB we have some recommendations for specific instrument use as well as sample drop off and reagent pickup.

ABI 7500 qPCR Instruments (Optional)

  • qPCR software – All users are allowed to have a copy of the qPCR analysis software on their own computers to allow analysis outside of LBB. If you do not have the software please place a request with the core lab. This will eliminate the need to analyze data in LBB at our workstations.
  • Running samples – LBB is happy to handle your qPCR plates and send data out. Please electronically provide us with the prepared Template file (save file as .edt). Then drop off the sample plate ready to go. We will place your samples on the instrument, run the assay and send you the processed data (.eds file) for analysis on your own local workstation. If you have questions please contact Derek Pouchnik (pooch@wsu.edu).

Sample Drop-off and Reagent Pickup – Next Gen DNA Sequencing, Sanger Sequencing and Fragment Analysis

  • Sample Drop off and Reagent Pickup – For researchers without access to our building, LBB is happy to accept samples and distribute DNA sequencing reagents at the front door of the Biotechnology Life Sciences building. Although we hope building access will soon be restored, in the meantime please contact LBB to coordinate a pickup and drop off time.
  • Please contact LBB at 509-335-1174, or e-mail core lab personnel: