LBB Services Offered
LBB provides a complete service for Affymetrix expression microarrays. We accept
total RNA and perform all necessary amplification and labeling reactions.
Labeled samples are then hybridized onto user specified Affymentrix microarrays
followed by array washing/staining and scanning. Data is then delivered via our
data server or secure file transfer. For a complete list of Affymetrix
expression microarrays available and an overview of their technology please
Click here for pricing
Sanger DNA Sequencing and DNA Fragment Analysis:
LBB currently runs and maintains a 3730 DNA Analyzer (Applied Biosystems). This
is a 48-capillary electorphoresis system designed for Sanger DNA sequencing and
DNA fragment analysis. The BigDye Terminator ready reaction mix from Applied
Biosystems is used for all DNA sequencing reactions. This can be acquired at the
core facility or purchased directly from Applied Biosystems. For a brief video
explanation of the Sanger Sequencing process go to :
The 3730 DNA Analyzer is also useful for DNA Fragment analysis. Samples of
fluorescently labeled DNA fragments are ran along with a size standard to
determine fragment sizes. We can accurately size fragments up to 1200 bp using
Applied Biosystems "Liz" size standards (Liz500, Liz600, and Liz1200). The
fluorescent label is generally incorporated by amplifying the DNA fragments
using a fluorescently labeled DNA primer. There are 4 different fluorescent
labels we can use with our instrument and they are 6-FAM, VIC, NED, and PET.
Primers with these labels are available from Applied Biosystems.
Click here for pricing
Next Generation Sequencing:
Pyrosequencing is currently performed on a Roche 454 FLX Titanium Genome
Sequencer. The long reads generated are best suited for de novo genome and
transcriptome assembly. In addition, adding molecular barcodes to samples allows
an extensive capacity to multiplex, making the 454 very useful for researchers
with a large number of samples as is often the case with amplicon analysis,
microbial community analysis and metagenomics. In spring we will have one of the
first Ion Torrent semi-conductor based sequencers, which will provide a million
100bp reads at low cost for researchers mapping expression data to reference
genomes, or other smaller data set needs. We work directly with investigators to
solve challenging sequencing problems.
Agilent 2100 Bioanalyzer:
The agilent 2100 bioanalyzer has become a very useful tool in analyzing RNA and
DNA samples. The Agilent 2100 Bioanalyzer is a microfluidics-based platform for
the analysis of DNA, RNA, and proteins. It delivers high-quality digital data in
30 minutes using lab-on-a-chip technology. We currently are running the RNA pico
and nano chips as well as the DNA 7500 and high sensitivity DNA chips. For more
information on the Agilent 2100 Bioanalyzer please visit the Agilent web site.
Mass Spectrometry and Proteomics:
A Bruker Esquire HCT trap mass spectrometry with a nanoflow HPLC is the main
proteomics instrumentation for protein identitfication of tryptic digest. An
Applied Biosystems 4800 Tof/Tof mass spec is also available for molecular weight
determination of whole proteins and other large molecules and for accurate mass
for small synthetic compounds in addition to mass spec fingerprinting of tryptic
digest and MS/MS on peptides for protein identification. More information on the
proteomics and fees can be found at:
Two Applied Biosystems 431 peptide synthesizers use Fmoc chemistry to synthesize
peptides on the 0.25mmole or 0.1 mmole scale. Other compounds using Fmoc
chemistry can also be synthesized using solid phase methods as well. Please
contact us for any of your custom Fmoc synthesis needs and we’ll see what we can
Real Time PCR:
Two Applied Biosystems 7500 Fast real time PCR instruments are available for
use. LBB maintains the instruments and assists in training users of these
instruments. Users must provide all necessary reagents and consumables. A sign
up sheet for the instruments is located in room 227 BLS. For more information
regarding real time PCR and the Applied Biosystem instruments please try the
There is currently no fee for the use of these instruments.
For your convenience LBB also has a Nanodrop Spectrometer (ND1000). The nanodrop
spectrophotometer is a small volume spectrophotometer (as little as 1ul) that
has proven to be very handy for many researchers.
There is currently no fee for the use if this instrument.