Office of Research

Laboratory of Biotechnology and Bioanalysis

LBB Services Offered


Affymetrix Microarrays:

LBB provides a complete service for Affymetrix expression microarrays. We accept total RNA and perform all necessary amplification and labeling reactions. Labeled samples are then hybridized onto user specified Affymentrix microarrays followed by array washing/staining and scanning. Data is then delivered via our data server or secure file transfer. For a complete list of Affymetrix expression microarrays available and an overview of their technology please visit http://www.affymetrix.com
Click here for pricing

Sanger DNA Sequencing and DNA Fragment Analysis:

LBB currently runs and maintains a 3730 DNA Analyzer (Applied Biosystems). This is a 48-capillary electorphoresis system designed for Sanger DNA sequencing and DNA fragment analysis. The BigDye Terminator ready reaction mix from Applied Biosystems is used for all DNA sequencing reactions. This can be acquired at the core facility or purchased directly from Applied Biosystems. For a brief video explanation of the Sanger Sequencing process go to : http://www.dnalc.org/resources/animations/cycseq.html

The 3730 DNA Analyzer is also useful for DNA Fragment analysis. Samples of fluorescently labeled DNA fragments are ran along with a size standard to determine fragment sizes. We can accurately size fragments up to 1200 bp using Applied Biosystems "Liz" size standards (Liz500, Liz600, and Liz1200). The fluorescent label is generally incorporated by amplifying the DNA fragments using a fluorescently labeled DNA primer. There are 4 different fluorescent labels we can use with our instrument and they are 6-FAM, VIC, NED, and PET. Primers with these labels are available from Applied Biosystems.
Click here for pricing

Next Generation Sequencing:

Pyrosequencing is currently performed on a Roche 454 FLX Titanium Genome Sequencer. The long reads generated are best suited for de novo genome and transcriptome assembly. In addition, adding molecular barcodes to samples allows an extensive capacity to multiplex, making the 454 very useful for researchers with a large number of samples as is often the case with amplicon analysis, microbial community analysis and metagenomics. In spring we will have one of the first Ion Torrent semi-conductor based sequencers, which will provide a million 100bp reads at low cost for researchers mapping expression data to reference genomes, or other smaller data set needs. We work directly with investigators to solve challenging sequencing problems.

Agilent 2100 Bioanalyzer:

The agilent 2100 bioanalyzer has become a very useful tool in analyzing RNA and DNA samples. The Agilent 2100 Bioanalyzer is a microfluidics-based platform for the analysis of DNA, RNA, and proteins. It delivers high-quality digital data in 30 minutes using lab-on-a-chip technology. We currently are running the RNA pico and nano chips as well as the DNA 7500 and high sensitivity DNA chips. For more information on the Agilent 2100 Bioanalyzer please visit the Agilent web site. http://nano.tm.agilent.com/index.cgi?CONTENT_ID=78

Mass Spectrometry and Proteomics:

A Bruker Esquire HCT trap mass spectrometry with a nanoflow HPLC is the main proteomics instrumentation for protein identitfication of tryptic digest. An Applied Biosystems 4800 Tof/Tof mass spec is also available for molecular weight determination of whole proteins and other large molecules and for accurate mass for small synthetic compounds in addition to mass spec fingerprinting of tryptic digest and MS/MS on peptides for protein identification. More information on the proteomics and fees can be found at: http://www.lbb2lab.wsu.edu/

Peptide Synthesis:

Two Applied Biosystems 431 peptide synthesizers use Fmoc chemistry to synthesize peptides on the 0.25mmole or 0.1 mmole scale. Other compounds using Fmoc chemistry can also be synthesized using solid phase methods as well. Please contact us for any of your custom Fmoc synthesis needs and we’ll see what we can do.

Real Time PCR:

Two Applied Biosystems 7500 Fast real time PCR instruments are available for use. LBB maintains the instruments and assists in training users of these instruments. Users must provide all necessary reagents and consumables. A sign up sheet for the instruments is located in room 227 BLS. For more information regarding real time PCR and the Applied Biosystem instruments please try the following link: http://www.appliedbiosystems.com/absite/us/en/home/applications-technologies/real-time-pcr.html
There is currently no fee for the use of these instruments.

NanoDrop Spectrophotometer:

For your convenience LBB also has a Nanodrop Spectrometer (ND1000). The nanodrop spectrophotometer is a small volume spectrophotometer (as little as 1ul) that has proven to be very handy for many researchers. http://www.nanodrop.com/
There is currently no fee for the use if this instrument.

Office of Research, PO Box 646525, Washington State University, Pullman WA 99164-1060, 509-335-1174, Contact Us