Sanger Sequencing

Sanger Sequencing is a method developed by Frederick Sanger in 1977 based on incorporation of fluorescently labeled dideoxynucleotides during DNA replication. It was the most widely used sequencing method for over 40 years, and is still widely used for non-NGS sequencing applications. Typical applications for Sanger Sequencing might include amplicon sequencing, SNP validation or confirming plasmid constructs. The high accuracy and ease of analysis make Sanger Sequencing an attractive technique for many researchers.

LBB employs an ABI 3730xl DNA Analyzer for our Sanger Sequencing enabling sequencing of hundreds of samples per day using BigDye V3.0 Cycle Sequencing chemistry from ThermoFisher Scientific.  

 

ABI 3730 DNA Analyzer

The ABI 3730 DNA Analyzer is a 48-capillary DNA sequencer and Genotyper that provides automated high throughput genetic analysis of DNA fragments. The 3730 can sequence 48 samples in parallel in two hours providing the capacity for analyzing hundreds of DNA samples per day. The instrument is fully automated from sample loading through data analysis. The 3730 is used for DNA fragment analysis such as microsatellites, SNP analysis, mutation detection, and Sanger Sequencing of plasmids and PCR products.

Sanger Sequencing Guidelines

DNA Sample Sheet

 

 

 

ABI 3730 Services: Sanger Sequencing

WSU Internal
within area or Federal:

WSU Internal
outside area or Non-Federal:

External
(non-profit):

Corporate:
96-well discount w/o PCR and Cleanup

$3.30

$3.59

$9.50

$10.00
96-well discount with PCR and Cleanup

$4.40

$4.78

$9.75

$10.25
No omega bead clean-up

$4.60

$5.00

$9.50

$10.00
Omega bead clean-up

$5.50

$5.98

$9.75

$10.25
PCR/Clean-up

$6.05

$6.58

$9.75

$10.25